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In a particular embodiment, the replication of claimed origin of replication might even more count on a approach, for instance transcription, to activate reported replication.
262、细菌噬菌体可选自长尾噬菌体科(非限制性地比如以下属：cam病毒、lika病毒、r4病毒、acadian病毒、cooper病毒、pg1病毒、pipefish病毒、rosebush病毒、brujita病毒、che9c病毒、hawkeye病毒、plot病毒、jersey病毒、k1g病毒、sp31病毒、lmd1病毒、una4病毒、bongo病毒、rey病毒、butters病毒、charlie病毒、redi病毒、baxter病毒、nymphadora病毒、bignuz病毒、fishburne病毒、phayonce病毒、kp36病毒、rogue1病毒、rtp病毒、t1病毒、tls病毒、ab18病毒、amigo病毒、anatole病毒、andromeda病毒、attis病毒、barnyard病毒、bernal13病毒、biseptima病毒、bron病毒、c2病毒、c5病毒、cba181病毒、cbast病毒、ceci病毒、che8病毒、chi病毒、cjw1病毒、corndog病毒、cronus病毒、d3112病毒、d3病毒、decurro病毒、demosthenes病毒、doucette病毒、e125病毒、eiau病毒、ff47病毒、gaia病毒、giles病毒、gordon病毒、gordtnk病毒、harrison病毒、hk578病毒、hk97病毒、jenst病毒、jwx病毒、kellezio病毒、korra病毒、l5病毒、lambda病毒、laroye病毒、liefie病毒、marvin病毒、mudcat病毒、n15病毒、nonag病毒、np1病毒、omega病毒、p12002病毒、p12024病毒、p23病毒、p70病毒、pa6病毒、pamx74病毒、tolerance病毒、pbi1病毒、pepy6病毒、pfr1病毒、phic31病毒、phicbk病毒、phieta病毒、phifel病毒、phijl1病毒、pis4a病毒、psa病毒、psimuna病毒、rdjl病毒、rer2病毒、sap6病毒、send513病毒、septima3病毒、seurat病毒、sextaec病毒、sfi11病毒、sfi21dt1病毒、sitara病毒、sk1病毒、slash病毒、smoothie病毒、soups病毒、spbeta病毒、ssp2病毒、t5病毒、tank病毒、tin2病毒、titan病毒、tm4病毒、tp21病毒、tp84病毒、tria病毒、trigintaduo病毒、vegas病毒、vendetta病毒、wbeta病毒、wildcat病毒、wizard病毒、woes病毒、xp10病毒、ydn12病毒和yua病毒)。
They ended up also reworked into MG1655 cells (s003): these strains ended up used to verify the titers obtained, For the reason that payloads should not be replicative from the absence with the primase protein supplied in trans.
In the method of the current invention, a nucleic acid of curiosity is shipped right into a specific receiver bacterial mobile of said microbiome or a gaggle of targeted receiver bacterial cells of mentioned microbiome, explained nucleic acid of fascination currently being comprised in a vector furnished by a donor bacterial mobile.
As made use of herein, the conditions “phagemid” and “phasmid” are equivalent and confer with a vector that derives from equally a plasmid plus a bacteriophage genome. A phagemid with the disclosure comprises a phage packaging web-site and an origin of replication (ori), as disclosed down below.
This is completely various for any bacterial ORI, as it would mean that It could be Energetic naturally and constitutively.
A independent team of mice colonized Together with the concentrate on pressure obtained just the buffer, to account for natural adjustments in colonization levels above enough time in the experiment.
24. The nucleic acid vector In keeping with embodiment 23, whereby said conditional origin of replication would be the primase on within the PICI of the Escherichia coli strain CFT073 or a by-product thereof.
Conditional Origin of Replication The vector of the creation comprises a conditional origin of replication that is inactive while in the focused receiver bacterial cell but is Energetic inside a donor bacterial mobile.
in a single embodiment, the focused receiver microorganisms are Bacteroides thetaiotaomicron and/or Bacteroides faecis.
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They are typically employed to select for recombination situations between a plasmid carrying this kind of origins and also the genome of the microbes of curiosity.
this kind of administration sorts might be sound, semi-solid or liquid, depending on the way 立即參與 and route of administration. one example is, formulations for oral administration could be provided having an enteric coating that will permit the vector, bacterial shipping auto or donor bacterial cell, from the formulation to resist the gastric setting and pass into your intestines. far more generally, vector formulations, bacterial shipping and delivery car or truck formulations or donor bacterial mobile formulations for oral administration could be suitably formulated for shipping into any desired Portion of the gastrointestinal tract. On top of that, suitable suppositories can be employed for shipping and delivery to the gastrointestinal tract. several pharmaceutically or cosmetically satisfactory carriers, diluents and excipients useful in pharmaceutical or veterinary or beauty compositions are identified to your experienced individual
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